{"title":"R I Freshney","description":null,"products":[{"product_id":"animal-cell-culture-book-r-i-freshney-9780199632138","title":"Animal Cell Culture","description":"This new edition takes account of the progress that has been made in the field since the publication of the first edition in 1986. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxicity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume should be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.","brand":"WoB","offers":[{"title":"GB \/ GOOD \/ INTERNAL","offer_id":49624399446289,"sku":"GOR003256425","price":0.0,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0784\/4072\/6801\/files\/0199632138.jpg?v=1751227384"},{"product_id":"culture-of-animal-cells-book-r-i-freshney-9780471453291","title":"Culture of Animal Cells","description":"This title is the most complete resource on the techniques, equipment,principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of \"Culture of Animal Cells\". In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: an all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols; new coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering; an expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment; and enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text. It also includes: fully updated references; the clearest, most consistent presentation of step-by-step protocols available; numerous diagrams, photographs, tables, and charts; and detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites. Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.","brand":"WoB","offers":[{"title":"US \/ GOOD \/ SBYB","offer_id":50155909153041,"sku":"CIN0471453293G","price":0.0,"currency_code":"GBP","in_stock":true},{"title":"US \/ WELL_READ \/ SBYB","offer_id":50328742560017,"sku":"CIN0471453293A","price":0.0,"currency_code":"GBP","in_stock":false},{"title":"GB \/ VERY_GOOD \/ INTERNAL","offer_id":50875072971025,"sku":"GOR006241446","price":0.0,"currency_code":"GBP","in_stock":true},{"title":"US \/ VERY_GOOD \/ SBYB","offer_id":52506398785809,"sku":"CIN0471453293VG","price":0.0,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0784\/4072\/6801\/files\/0471453293.jpg?v=1751198008"},{"product_id":"culture-of-animal-cells-book-r-i-freshney-9780471348894","title":"Culture of Animal Cells","description":"The fourth edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting. The advantages of tissue culture go beyond control of the physiochemical environment and physiological conditions as shown in the comprehensive coverage of tissue culture topics, both organ culture and cell culture, provided in this manual. A wide range of essential information from basic to specialized procedures is presented, highlighting advantages and limitations, and illustrating the properties of different types of culture. This crucial reference for cell culture techniques includes:New Atlas of Cells section in full-color presentation Extended coverage of molecular techniques, scale-up, and serum-free medium New chapter on problem solving Photographs of cell lines, contaminations, and equipment Clear and concise tables and charts Educated recommendations on safety issues, ethical consent, and ownership Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference.","brand":"WoB","offers":[{"title":"US \/ GOOD \/ SBYB","offer_id":50445265502481,"sku":"CIN0471348899G","price":0.0,"currency_code":"GBP","in_stock":false},{"title":"US \/ VERY_GOOD \/ SBYB","offer_id":50762220699921,"sku":"CIN0471348899VG","price":0.0,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0784\/4072\/6801\/files\/0471348899.jpg?v=1751167148"},{"product_id":"culture-of-animal-cells-book-r-i-freshney-9780471589662","title":"Culture of Animal Cells","description":"In this edition, the author provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of animal cells. Containing step-by-step laboratory procedures, detailed instructions for reagent preparation, and information on latest specialized equipment and supplies, this laboratory manual includes over 350 new references, 13 new protocols plus recent methods and expanded protocols in areas of new technology such as antibody staining and cytotoxicity assay.","brand":"WoB","offers":[{"title":"GB \/ VERY_GOOD \/ INTERNAL","offer_id":52413163372817,"sku":"GOR004871305","price":0.0,"currency_code":"GBP","in_stock":false},{"title":"US \/ WELL_READ \/ SBYB","offer_id":53267118653713,"sku":"CIN0471589667A","price":0.0,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0784\/4072\/6801\/files\/9780471589662.jpg?v=1758825216"}],"url":"https:\/\/www.worldofbooks.com\/collections\/author-books-by-r-i-freshney.oembed","provider":"World of Books ","version":"1.0","type":"link"}