Advanced Technologies for Protein Complex Production and Characterization by M Cristina Vega

Advanced Technologies for Protein Complex Production and Characterization by M Cristina Vega

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Dr. M. Cristina Vega is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance. 

SKU	Nicht verfügbar
ISBN 13	9783319272146
ISBN 10	3319272144
Titel	Advanced Technologies for Protein Complex Production and Characterization
Autor	M Cristina Vega
Serie	Advances In Experimental Medicine And Biology
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Bindungsart	Hardback
Verlag	Springer International Publishing AG
Erscheinungsjahr	2016-05-20
Seitenanzahl	382
Hinweis auf dem Einband	Die Abbildung des Buches dient nur Illustrationszwecken, die tatsächliche Bindung, das Cover und die Auflage können sich davon unterscheiden.
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