Are You Up for The Challenge? by Rod Hairston

Are You Up for The Challenge? by Rod Hairston

Parafibromin (PFM) is a recently identified tumour suppressor that is lost in various types of cancer, including parathyroid, renal, breast and gastric cancer. PFM is a component of the Paf1 complex (Paf1C), which is thought to have key roles at various stages of the gene expression pathway. In the present work, we identified the molecular architecture of the human Paf1C (hPaf1C). We found that the C-terminus of PFM interacts with the hPaf1C. Since almost all known clinically relevant PFM mutations effect the expression of the C-terminal part of PFM, our findings provide evidence that the tumour supressor function of PFM is dependent on the hPaf1C. Interestingly, we further found that the same domain on PFM that is responsible for binding to -Catenin mediates the interaction to Paf1 and Ski8 and report for the first time that PFM is able to dimerize in vitro and that it is the only hPaf1C component that directly interacts with URI. PFM-/- MEFs generated show a decreased expression of the PFM target genes Igf1, Igf2, Hmg1 and Igfbp4 but an unaltered activity of the PI3K-pathway. Last but not least, our data suggest that Cre-mediated deletion of PFM in MEFs causes cell death.

SKU	Nicht verfügbar
ISBN 13	9780979262005
ISBN 10	0979262003
Titel	Are You Up for The Challenge?
Autor	Rod Hairston
Buchzustand	Nicht verfügbar
Bindungsart	Paperback
Verlag	Invictus International
Seitenanzahl	178
Hinweis auf dem Einband	Die Abbildung des Buches dient nur Illustrationszwecken, die tatsächliche Bindung, das Cover und die Auflage können sich davon unterscheiden.