Biological Low-Voltage Scanning Electron Microscopy by James Pawley

Biological Low-Voltage Scanning Electron Microscopy by James Pawley

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

From the reviews:

"Topics ranging from the early development of the scanning electron microscope (SEM) to some of the latest low-voltage and cryo techniques are reviewed in detail… this is a very useful volume for those wishing to learn, or refresh their memory, in the context of getting the best out of an SEM, and in particular the field emission SEM (FESEM)." (Iolo Ap Gwynn, infocus Magazine, Issue 12, December, 2008)

“Biological Low-voltage Scanning Electron Microscopy is an excellent and timely collection, written by many of the recognized experts in the subject … . Strongly recommended.” (Ultramicroscopy, Vol. 109, June, 2009)

Heide Schatten is Professor at the University of Missouri, Columbia, USA. She is a cell biologist with research focused on cytoskeletal regulation in various cell systems and on cytoskeletal dysfunctions that play a role in disease such as cancer.

Her interest in cancer research was stimulated by father and sister physicians who as clinicians were faced with treating incurable diseases for which research on cell and molecular levels had not yet advanced to our current state of knowledge. She pursued her interest in research and received her M.S. and Ph.D. degrees from the Karl-Ruprecht University in Heidelberg, Germany, and performed her Ph.D. research studies at the German Cancer Research Center in Heidelberg to explore the mechanisms leading to abnormal cell divisions in cancer and to test drugs that prevent abnormal cell proliferation. She performed pre-and postdoctoral studies at the University of California, Berkeley, and entertained numerous collaborations with colleagues in the USA, Canada, Europe, China, and Latin America in the fields of reproduction and cancer using cell and molecular biology approaches. She has given numerous presentations in these and other countries. Her studies also included collaborations with NASA scientists and experiments aboard the Space Shuttle Endeavour to examine the effects of spaceflight on centrosome-cytoskeletal regulation during development. She also holds an M.S. degree in music from the School of Music in Heidelberg.

She is a member of the American Society for Cell Biology, American Association for the Advancement of Science, and Microscopy Society of America. She has received numerous awards including grant awards from NASA, NIH, and NSF. She has published over 210 papers, 13 book chapters and edited several special topics journal issues and 9 books with several more in progress.

SKU	Nicht verfügbar
ISBN 13	9781489995841
ISBN 10	1489995846
Titel	Biological Low-Voltage Scanning Electron Microscopy
Autor	James Pawley
Buchzustand	Nicht verfügbar
Bindungsart	Paperback
Verlag	Springer-Verlag New York Inc.
Erscheinungsjahr	2014-09-13
Seitenanzahl	317
Hinweis auf dem Einband	Die Abbildung des Buches dient nur Illustrationszwecken, die tatsächliche Bindung, das Cover und die Auflage können sich davon unterscheiden.
Hinweis	Nicht verfügbar