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PCR 2 By Edited by M. J. McPherson

PCR 2
by Edited by M. J. McPherson

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PCR is now one of the most widely used basic molecular biology techniques. This volume sets out to address some of the new applications of PCR, including direct sequencing, mRNA quantitation and expression, genomic DNA mapping and mutation analysis.
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PCR 2 Summary


PCR 2: A Practical Approach by Edited by M. J. McPherson

PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis.

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PCR 2 Reviews


This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. * Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6 *

Table of Contents


1. Optimizing PCR ; 2. Use of specialty phosphoramidites in PCR ; 3. Chemical methods for 5' non-isotopic labelling of PCR probes and primers ; 4. Solid-phase PCR ; 5. Solid-phase sequencing of PCR products ; 6. cDNA cloning by RT - PCR ; 7. Quantification of DNA and RNA by PCR ; 8. PCR MIMICS: competitive DNA fragments for use in quantitative PCR ; 9. In vitro expression of proteins from PCR products ; 10. PCR-based approaches to human genome mapping ; 11. Fingerprinting of DNA and RNA using arbitrarily primed PCR ; 12. Mutational analysis ; 13. Mutational analysis: new mutations ; 14. Linear amplification for the in virto study of ligand/DNA interactions ; 15. Ligand-mediated PCR

Additional information

GOR002840000
PCR 2: A Practical Approach by Edited by M. J. McPherson
Edited by M. J. McPherson
Practical Approach Series
Used - Very Good
Paperback
Oxford University Press
1995-06-01
358
0199634246
9780199634248
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a used book - there is no escaping the fact it has been read by someone else and it will show signs of wear and previous use. Overall we expect it to be in very good condition, but if you are not entirely satisfied please get in touch with us.