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Membrane Proteins By Giovanna Ghirlanda

Membrane Proteins by Giovanna Ghirlanda

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Summary

Focusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems.

This book will be specially printed for your order, which means it may take a little longer to arrive (5 days). We do this to cut down on waste & to help protect our planet.

Membrane Proteins Summary

Membrane Proteins: Folding, Association, and Design by Giovanna Ghirlanda

Focusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems. The volume is divided into four detailed sections, covering association of transmembrane helices, interactions with the lipid bilayer, NMR methods, as well as a variety of engineering approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Membrane Proteins: Folding, Association, and Design serves as an ideal guide for researchers reaching for the tantalizing possibility of designing novel membrane proteins with tailored functionality.

Table of Contents

Part I: Association of Transmembrane Helices

1. Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments

Lidia Cristian and Yao Zhang

2. Measurement of Transmembrane Peptide Interactions in Liposomes Using Foerster Resonance Energy Transfer (FRET)

Ambalika Khadria and Alessandro Senes

3. Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping

Heedeok Hong, Yu-Chu Chang, and James U. Bowie

4. Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes

Lydia Tome, Dominik Steindorf, and Dirk Schneider

Part II: Interactions with the Lipid Bilayer

5. Analyzing the Effects of Hydrophobic Mismatch on Transmembrane -Helices Using Tryptophan Fluorescence Spectroscopy

Gregory A. Caputo

6. Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure

Heather E. Findlay and Paula J. Booth

7. Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes

Andrei L. Lomize and Irina D. Pogozheva

Part III: NMR Methods

8. Membrane Protein Structure Determination: Back to the Membrane

Yong Yao, Yi Ding, Ye Tian, Stanley J. Opella, and Francesca M. Marassi

9. On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides

Fernando Porcelli, Ayyalusamy Ramamoorthy, George Barany, and Gianluigi Veglia

Part IV: Engineering Approaches

10. Prediction and Design of Outer Membrane Protein-Protein Interactions

Vikas Nanda, Daniel Hsieh, and Alexander Davis

11. Design of Transmembrane Peptides: Coping with Sticky Situations

Arianna Rath and Charles M. Deber

12. Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors

Matthew W. Lluis and Hang Yin

13. Fluorination in the Design of Membrane Protein Assemblies

Vijay M. Krishnamurthy and Krishna Kumar

Additional information

NLS9781493960217
9781493960217
1493960210
Membrane Proteins: Folding, Association, and Design by Giovanna Ghirlanda
New
Paperback
Humana Press Inc.
20160823
246
null null null null null null null null null null
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