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Planar Cell Polarity Kursad Turksen

Planar Cell Polarity By Kursad Turksen

Planar Cell Polarity by Kursad Turksen


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Summary

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.

Planar Cell Polarity Summary

Planar Cell Polarity: Methods and Protocols by Kursad Turksen

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world. As a volume in the highly successful Methods in Molecular Biology (TM) series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.

Table of Contents

1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris

2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell

Bertrand Jauffred and Yohanns Bellaiche

3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus

Yingqun Wang

4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity

David Neff, Justin Hogan, and Simon Collier

5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation

Douglas C. Weiser and David Kimelman

6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation

Jason R. Jessen

7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation

Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han

8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar

9. Gene Loss-of-Function and Live Imaging in Chick Embryos

Anne C. Rios, Christophe Marcelle, and Olivier Serralbo

10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation

Courtney Mezzacappa, Yuko Komiya, and Raymond Habas

11. Convergent Extension Analysis in Mouse Whole Embryo Culture

Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, and Andrew J. Copp

12. Analysis of PCP Defects in Mammalian Eye Lens

Yuki Sugiyama and John W. McAvoy

13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea

Helen May-Simera and Matthew W. Kelley

14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

Lisa Fujimura and Masahiko Hatano

15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

Kristi LaMonica and Laura Grabel

16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami

17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

Shinji Matsumoto and Akira Kikuchi

18. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

Makoto Matsuyama and Akihiko Shimono

19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

Daniel J. Jagger and Andrew Forge

20. Morphometric Analysis of Centrosome Position in Tissues

Hester Happe, Emile de Heer, and Dorien J.M. Peters

Additional information

NLS9781493959068
9781493959068
1493959069
Planar Cell Polarity: Methods and Protocols by Kursad Turksen
New
Paperback
Humana Press Inc.
2017-04-30
262
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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