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Bacteriophages By Martha R. J. Clokie

Bacteriophages by Martha R. J. Clokie

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$204.99
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Summary

This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models.

Bacteriophages Summary

Bacteriophages: Methods and Protocols, Volume IV by Martha R. J. Clokie

This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The chapters in this book cover molecular topics such as PhageFISH for monitoring phage infections at single cell level; the analysis of phage-host protein-protein interactions using Strep-tag (R) II purifications; and also application driven chapters including 'duckweed (Lemna minor) and alfalfa (Medicago sativa) as bacterial infection model systems'. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and thorough, Bacteriophages: Methods and Protocols, Volume IV is a valuable resource for both established and novice phage scientists.

Bacteriophages Reviews

"The book describes the functional elucidation of bacteriophages in the current era, as well as the drive towards application-driven research. ... this is a very interesting resource for protocols regarding phages and their use for therapy. I am going to include it in my reference material for my own students working on phages in relation to the gut microbiota." (Koen Venema, Beneficial Microbes, Vol. 10 (3), 2019)

About Martha R. J. Clokie

N/A

Table of Contents

Preface...

Table of Contents...

Contributing Authors...


1. PhageFISH for Monitoring Phage Infections at Single Cell Level

Cristina Moraru and Jimena Barrero-Canosa


2. Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria

Mariana Piuri and Grahan Hatfull


3. Engineering Bacteriophage-Based Biosensors

Daniel Brownell, John King, Brian Caliando, Lada Sycheva, and Michael Koeris


4. Introduction of Phage Genome into Escherichia coli by Electroporation

Nika Janez, Sasa Haberl Meglic, Karel Flisar, Damijan Miklavcic, and Matjaz Peterka


5. Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1

Charles R. Stewart


6. Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA

Laura J. Marinelli, Mariana Piuri, and Graham F. Hatfull


7. Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography

Krystyna Dabrowska


8. Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes

Hany Anany, Luba Brovko, Denis Arutyunov, Nilufar Poshtiban, Amit Singh, Upasana Singh, Michael Brook, Christine Szymanski, Stephane Evoy, and Mansel Griffiths


9. Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis

Lorena Rodriguez-Rubio, David M. Donovan, Beatriz Martinez, Ana Rodriguez, and Pilar Garcia


10. Analyzing Phage-Host Protein-Protein Interactions using Strep-tag (R) II Purifications

Jeroen De Smet, Hanne Hendrix, and An Van den Bossche


11. Techniques to Assess Phage/Biofilm Interaction

Diana Vilas Boas, Carina Almeida, Nuno Azevedo, Sanna Sillankorva, and Joana Azeredo


12. Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages

Hanne Hendrix, Ines Staes, Abram Aertsen, and Jeroen Wagemans


13. The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis

Fatima Kamal, Danielle L. Peters, Jaclyn G. McCutcheon, Gary B. Dunphy, and Jonathan J. Dennis


14. Use of a Silkworm Larva Model in Phage Therapy Experiments

Jumpei Uchiyama, Iyo Takemura-Uchiyama, and Shigenobu Matsuzaki


15. Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages

Hye-Jeong Jang, Hee-Won Bae, and You-Hee Cho


16. Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems

Fatima Kamal, Alina Radziwon, Carly M. Davis, and Jonathan J. Dennis


17. Quantitating Phage Efficacy in ready-To-Eat Meats

Andrew Chibeu and Sampathkumar Balamurugan


18. Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy

Angelina Trotereau and Catherine Schouler


Index...

Additional information

GOR011058680
9781493989393
1493989391
Bacteriophages: Methods and Protocols, Volume IV by Martha R. J. Clokie
Used - Like New
Hardback
Humana Press Inc.
2018-12-20
216
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
The book has been read, but looks new. The book cover has no visible wear, and the dust jacket is included if applicable. No missing or damaged pages, no tears, possible very minimal creasing, no underlining or highlighting of text, and no writing in the margins

Customer Reviews - Bacteriophages