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Patch-Clamp Methods and Protocols By Marzia Martina

Patch-Clamp Methods and Protocols by Marzia Martina

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Patch-Clamp Methods and Protocols Summary

Patch-Clamp Methods and Protocols by Marzia Martina

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Patch-Cla

mp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

Patch-Clamp Methods and Protocols Reviews

This is a very good up to date 'how to' manual with dozens of unique protocols regarding single channel, whole cell, and network patch recordings. ... This atlas and textbook is a good choice for 'patchers', neurohysiologists, biophysicists, students, fellows and 'cell' molecular physiologists. (Joseph J. Grenier,, September, 2015)

Table of Contents

1. History of Electrophysiology and the Patch-Clamp

Alexej Verkhratysky and Vladimir Parpura

Part I. Pharmacology

2. Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications

Nate Glasgow and Jon W. Johnson

3. Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers

Chris G. Parsons and Kate E. Gilling

4. State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening

Sonja Stoelzle-Feix

5. Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch (R) 96 Using Instant Frozen Cells

Liudmila Polonchuk

6. Planar Patch Clamp for Neuronal Networks-Considerations and Future Perspectives

Alessandro Bosca, Marzia Martina, and Christophe Py

Part II. Physiology

7. Methods for the Study of Synaptic Receptor Functional Properties

Enrica Maria Petrini and Andrea Barberis

8. Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity

Maria Toledo-Rodriguez and Henry Markram

9. Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique

Romain Lefebvre, Sandrine Pouvreau, Claude Collet, B

runo Allard, and Vincent Jacquemond

10. Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability

Joshua L. Plotkin and James D. Surmeier

11. Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices

Giovanni Russo and Stefano Taverna

12. Outside-Out 'Sniffer-patch' Clamp Technique for in situ Measures of Neurotransmitter Release

Emilie Muller-Chretien

13. A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices

Cary Soares, Kevin F.H. Lee, Denise Cook, and Jean-Claude Beique

14. Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics

Jonathan T. Ting, Tanya L. Daigle, Qian Chen, and Guoping Feng

15. Synaptic Connectivity in Engineered Neuronal Networks

Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das, and James J. Hickman

16. Modeling of Action Potential Generation in NG108-15 Cells

Peter Molnar and James J. Hickman

17. Whole-Cell Patch-Clamp Recordings in Freely Moving Animals

Albert K. Lee, Jerome Epsztein, and Michael Brecht

Part III. Biophysics

18. Pressure-Polished Borosilicate Pipettes are Universal Sealer Yielding Low Access Resistance and Efficient Intracellular Perfusion

Marco Aquila, Mascia B

enedusi, Anna Fasoli, and Giorgio Rispoli

19. Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes

Romain Guinamard, Thomas Hof, and Laurent Salle II

20. Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents

Geza Berecki, Arie O. Verkerk, Antoni C.G. van Ginneken, and Ronald Wilders

21. Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI

Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White, and Alan D. Dorval

22. Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes

Thierry Cens and Pierre Charnet

23. Principles of Single-Channel Kinetic Analysis

Feng Qin

Additional information

Patch-Clamp Methods and Protocols by Marzia Martina
Humana Press Inc.
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