PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory
1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory
Monica V. Cunha And Joao Inacio
2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice
Alicia Aranaz
3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory
Joseph Kozlovac And Beverly Schmitt
4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes
Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari
5. Biological Specimen Collection And Processing for Molecular Analysis
Susan Sanchez And Holly Sellers
6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory
Nick Saunders and Ian Sharp
PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings
7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals
Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sandor Belak
8. Real Time Reverse Transcriptase PCR For The Detection Of Bluetongue Virus
Carrie Batten, Lorraine Frost, And Chris Oura
9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues
Pedro Costa, Isabel Couto, Miguel Viveiros, and Joao Inacio
10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants
Florian Roeber, Aaron R. Jex, And Robin B. Gasser
11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches
Irene R. Grant And Linda D. Stewart
12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique
Hatem Soliman, Mona Saleh, And Mansour El-Matbouli
13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches
Jacinto Gomes And Joao Inacio
14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection
Ana Hurtado
15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp.
Christiane Schnee and Konrad Sachse
16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues
Maria Ballester and Fernando Rodriguez
17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections
Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and Mette Boye
18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry
Joachim Frey and Peter Kuhnert
19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases
Mona Saleh, Hatem Soliman, and Mansour El-Matbouli
20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens
Fernando Teles And Luis Pina Fonseca
PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens
21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms
Konrad Sachse and Petra Moebius
22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis
Ana Claudia Carreira and Monica V. Cunha
23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci
Ilda Santos-Sanches, Lelia Chambel, and Rogerio Tenreiro
24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species
Giuliano Garofolo
25. Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains
Ahmed Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl
26. Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis
Sylviane Derzelle
27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex
Ana Botelho, Ana Canto, Celia Leao, and Monica V. Cunha
28. Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples
Konrad Sachse and Anke Ruettger
29. Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array
Simon Thierry and Sylviane Derzelle
PART IV - Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction With the Host
30. Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?
Steven Van Borm, Sandor Belak, Graham Freimanis, Alice Fusaro, Fredrik Granberg, Dirk Hoeper, Donald P. King, Isabella Monne, Richard Orton, and Toon Rosseel
31. Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions
Cinzia Cantacessi, Andreas Hofmann, Bronwyn E. Campbell, and Robin B. Gasser
32. Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina
Ana Domingos, Sandra Antunes, Margarita Villar, and Jose de la Fuente33. Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees
Fredrik Granberg, Oskar E. Karlsson, and Sandor Belak
34. Proteomics Characterization Of Tick-Host-Pathogen Interactions
Marina Popara, Margarita Villar, and Jose de la Fuente
