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Neisseria meningitidis By Myron Christodoulides

Neisseria meningitidis by Myron Christodoulides

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Neisseria meningitidis Summary

Neisseria meningitidis: Advanced Methods and Protocols by Myron Christodoulides

Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.

Table of Contents

1. Neisseria meningitidis: Biology, Microbiology, and Epidemiology

Nadine G. Rouphael and David S. Stephens

2. Classification and Pathogenesis of Meningococcal Infections

Petter Brandtzaeg and Marcel van Deuren

3. Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology

Jens Kjolseth Moller

4. Generating Knock-Out and Complementation Strains of Neisseria meningitides

Vincent van Dam and Martine P. Bos

5. Identification and Functional Characterization of sRNAs in Neisseria meningitides

Yvonne Pannekoek and Arie van der Ende

6. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

Muhammad Saleem, Jeremy Moore, and Jeremy P. Derrick

7. Genome-Scale Metabolic Models: Reconstruction and Analysis

Gino J.E. Baart and Dirk E. Martens

8. TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome

Karsten Kuhn, Christian Baumann, Jan Tommassen, and Thorsten Prinz

9. Meningococcal Ligands and Molecular Targets of the Host

Darryl J. Hill and Mumtaz Virji

10. In Vivo Imaging of Meningococcal Disease Dynamics

Hong Sjoelinder and Ann-Beth Jonsson

11. Methods for Studying Neisseria meningitidis Biofilms

Michael A. Apicella, Jianqiang Shao, and R. Brock Neil

12. A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress

Magali Soyer and Guillaume Dumenil

13. Techniques to Measure Pilus Retraction Forces

Nicolas Biais, Dustin Higashi, Magdalene So, and Benoit Ladoux

14. Human Dendritic Cell Culture and Bacterial Infection

Hannah E. Jones, Nigel Klein, and Garth L.J. Dixon

15. Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions

Jessmi M.L. Ling, Leslie Silva, David C. Schriemer, and Anthony B. Schryvers

16. Visualizing PAMP-PRR Interactions Using Nanoscale Imaging

Kathy Triantafilou and Martha Triantafilou

17. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

Biju Joseph, Matthias Frosch, Christoph Schoen, and Alexandra Schubert-Unkmeir

18. The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria

Nigel J. Saunders and John K. Davies

19. Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins

Paola Massari and Lee M. Wetzler

20. Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

Jeannette N. Williams, Myron Christodoulides, and John E. Heckels

21. Antigen Identification Starting from the Genome: A Reverse Vaccinology Approach Applied to MenB

Emmanuelle Palumbo, Luigi Fiaschi, Brunella Brunelli, Sara Marchi, Silvana Savino, and Mariagrazia Pizza

22. A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens

Jason Rice and Myron Christodoulides

Additional information

Neisseria meningitidis: Advanced Methods and Protocols by Myron Christodoulides
Humana Press Inc.
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