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HDAC/HAT Function Assessment and Inhibitor Development By Oliver H. Kramer

HDAC/HAT Function Assessment and Inhibitor Development by Oliver H. Kramer

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Summary

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro.

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HDAC/HAT Function Assessment and Inhibitor Development Summary

HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols by Oliver H. Kramer

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

Table of Contents

Part I: Analysis of Cellular Processes that Are Regulated by HDACs

1. Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells

Anja Goeder, Nisintha Mahendrarajah, and Oliver H. Kramer


2. Assessment of HDACi-Induced Protein Cleavage by Caspases

Fabian Treude, Tobias Gladbach, Jacqueline Plaster, and Joerg Hartkamp


3. Assessment of HDACi-Induced Cytotoxicity

Lisa Blumel, Christian Marx, Marie Kuhne, and Jurgen Sonnemann


4. Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization

Britta Unruhe-Knauf and Shirley K. Knauer


5. Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay

Sonja Mertsch and Solon Thanos


6. Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation

Darren M. Hutt, Daniela Martino Roth, Christelle Marchal, and Marion Bouchecareilh


7. Effects of HDACi on Immunological Functions

Rene Winkler and Christian Kosan


8. DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication

Teodora Nikolova, Anja Goeder, Ann Parplys, and Kerstin Borgmann


9. Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination

Andrea Krumm and Wynand P. Roos


Part II: Primary Cell Systems and In Vivo Models to Assess HDAC Functions


10. Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research

Annette Romanski and Gesine Bug


11. Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay

Katharina Wolf and Susanne Strand


12. Purification and Analysis of Male Germ Cells from Adult Mouse Testis

Thierry Buchou, Minjia Tan, Sophie Barral, Anne-Laure Vitte, Sophie Rousseaux, Juan Arechaga, and Saadi Khochbin


13. Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions

Astrid Hagelkruys, Mirjam A. Moser, and Christian Seiser


14. Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System

Sieglinde Bayer and Matthias Wirth


15. Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors

Daniel Nettersheim, Sina Jostes, and Hubert Schorle


16. Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi

Christina Susanne Mullins, Stephanie Bock, Mathias Krohn, and Michael Linnebacher


Part III: Assessment of HDAC-Dependent Effects on Signaling Cascades in Primary and Permanent Cells


17. Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment

Dagmar Hildebrand and Katharina F. Kubatzky


18. Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry

David Olagnier, Cindy Chiang, and John Hiscott


19. Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP)

Sophia Pinz and Anne Rascle


20. Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases

Benjamin Y. Owusu and Lidija Klampfer


21. Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells

Jing Jiao, Rongxiang Han, Wayne W. Hancock, and Ulf H. Beier


Part IV: PTMs Controlling Acetylation Balances and HDAC Functions


22. Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases

Jan Hagenbucher, Hilda Stekman, Alfonso Rodriguez-Gil, Michael Kracht, and M. Lienhard Schmitz


23. Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry

Martin Wieczorek, Karl-Heinz Guhrs, and Thorsten Heinzel


24. Assessing the Role of Paralog-Specific Sumoylation of HDAC1

Simona Citro and Susanna Chiocca


25. Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques

Tobias Wagner, Maren Godmann, and Thorsten Heinzel


Part V: Improved, Novel, and Specific HDACi


26. How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot

Mandy Beyer, Nicole Kiweler, Siavosh Mahboobi, and Oliver H. Kramer


27. Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors

Gonzalo Lopez and Raphael E. Pollock


28. Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors

Katrin Noack and Oliver H. Kramer


29. Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles

Dorle Hennig and Diana Imhof


30. Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly d, l-lactide-co-glycolide Polymeric Nanoparticles by Nanoprecipitation Method

Vilwanathan Ravikumar and Renu Sankar

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31. Generation and Assessment of Fusions Between HDACi and TKi

Siavosh Mahboobi, Bernadette Pilsl, and Andreas Sellmer

Additional information

NLS9781493982264
9781493982264
1493982265
HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols by Oliver H. Kramer
New
Paperback
Humana Press Inc.
2018-06-20
415
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
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