Cart
  • Free Shipping on all orders in Australia
  • Over 7 million books in stock
  • Proud to be B-Corp
  • We aim to be carbon neutral by 2022
  • Over 120,000 Trustpilot reviews
Item 1 of 0
Viral Vectors for Gene Therapy By Otto-Wilhelm Merten

Viral Vectors for Gene Therapy by Otto-Wilhelm Merten

Condition - New
$224.39
Only 2 left

Summary

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.

Viral Vectors for Gene Therapy Summary

Viral Vectors for Gene Therapy: Methods and Protocols by Otto-Wilhelm Merten

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular Biology (TM) series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Viral Vectors for Gene Therapy Reviews

From the reviews:

Intended for practitioners involved in the preclinical and clinical applications of gene therapy vectors. ... students, scientists, and physicians specializing in gene therapy will find this book extremely useful. ... Given the rapid advances in the field of gene therapy, this book is essential, providing useful information on the methodologies that involve virus production, transferring protocols, and evaluation of the efficacy of gene products and other important methodological concepts in gene therapy. (Omer Iqbal, Doody's Review Service, September, 2011)

Table of Contents

1. Introduction to Viral Vectors

James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai

2. Introduction to Gene Therapy: A Clinical Aftermath

Patrice P. Denefle

3. Host Cells and Cell Banking

Glyn N. Stacey and Otto-Wilhelm Merten

4. Overview of Current Scalable Methods for Purification of Viral Vectors

Maria Mercedes Segura, Amine Kamen, and Alain Garnier

5. Methods to Construct Recombinant Adenovirus Vectors

Miguel Chillon and Ramon Alemany

6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus

Edwige Dormond and Amine A. Kamen

7. Manufacturing of Retroviruses

Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha

8. Lentiviral Vectors

Marc Giry-Laterriere, Els Verhoeyen, and Patrick Salmon

9. Adeno-Associated Viruses

Mauro Mezzina and Otto-Wilhelm Merten

10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2

Haifeng Chen

11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses

J. Fraser Wright and Olga Zelenaia

12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells

Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mahoenen, and Seppo Yla-Herttuala

13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors

Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein

14. Manufacture of Measles Viruses

Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel

15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model

Cecilia Frecha, Floriane Fusil, Francois-Loic Cosset, and Els Verhoeyen

16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)

Kolja Gelse and Holm Schneider

17. Ethical Consideration

Michael Fuchs

18. Clinical Trials of GMP Products in the Gene Therapy Field

Kathleen B. Bamford

Additional information

NPB9781617790942
9781617790942
161779094X
Viral Vectors for Gene Therapy: Methods and Protocols by Otto-Wilhelm Merten
New
Hardback
Humana Press Inc.
20110519
450
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

Customer Reviews - Viral Vectors for Gene Therapy