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Malaria By Robert Menard

Malaria by Robert Menard

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Malaria Summary

Malaria: Methods and Protocols by Robert Menard

Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers.

Table of Contents

Part I. Parasite Culturing

1. In vitro Culturing P. falciparum Erythrocytic Stages

Alexander G. Maier and Melanie Rug

2. Production of Plasmodium falciparum Gametocytes in vitro

Kathryn Shaw Saliba and Marcelo Jacobs-Lorena

3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes

Anil K. Ghosh and Marcelo Jacobs-Lorena

4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages

Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken

5. Laboratory Maintenance of Rodent Malaria Parasites

Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden

Part II. Genome Manipulation

6. Transfection of Plasmodium falciparum

Melanie Rug and Alexander G. Maier

7. Transfection of Rodent Malaria Parasites

Nisha Philip, Rachael Orr, and Andrew P. Waters

8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNA

Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker

9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database

Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse

10. Transient Transfection of Plasmodium vivax Blood-stage Parasites

Cecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer

11. Silencing of Genes and Alleles by RNAi in Anopheles gambiae

Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stephanie A. Blandin

Part III. 'OMIC' Techniques

12. Analysis of Variant Gene Family Expression by Quantitative PCR

Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira

13. DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites

Zbynek Bozdech, Sachel Mok, and Archna P. Gupta

14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform

Heather J. Painter, Lindsey M. Altenhofen, Bjoern F.C. Kafsack and Manuel Llinas

15. Transcriptome Analysis using RNA-seq

Wieteke A.M. Hoeijmakers, Richard Bartfai, and Hendrik G. Stunnenberg

16. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium

Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig

17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis

Kellen L. Olszewski and Manuel Llinas

Part IV. Red Blood Cell Stages

18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum

David T. Riglar and Jake Baum

19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites

Shailja Singh and Chetan E. Chitnis

20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability

Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Genevieve Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet

21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events

Ang Li, Laurent Renia, Chwee Teck Lim, and Bruce Russell

22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System

Anand Srivastava, Yves Durocher, and Benoit Gamain

23. Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium

Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf

24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum

Liliana Mancio-Silva and Artur Scherf

25. Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents

Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard

Part V. Pre-Erythrocytic Stages

26. Scoring Sporozoite Motility

Allison F. Carey, Robert Menard, and Daniel Y. Bargieri

27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry

Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota

28. Imaging Sporozoite Cell Traversal in the Liver of Mice

Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Menard, and Rogerio Amino

29. Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes

Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler

30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging

Takeshi Annoura, Severine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan

Part VI. Immunity to Plasmodium

31. Activation of Human NK Cells by Plasmodium-infected Red Blood Cells

Amir Horowitz and Eleanor M. Riley

32. Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines

Ian Cockburn

33. Development and Use of TCR Transgenic Mice for Malaria Immunology Research

Yun-Chi Chen and Fidel Zavala

34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination

Katherine L. Doll, Noah S. Butler, and John T. Harty

Part VII. Controlling Plasmodium Infection

35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter Parasites

Jing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard

36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry

Anthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale

37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure

Danielle I. Stanisic and Michael F. Good

38. Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites

Ashley M Vaughan and Stefan H I Kappe

39. Vaccination using Normal Live Sporozoites Under Drug Treatment

Laurent Renia, Anne Charlotte Gruner, Marjorie Mauduit, and Georges Snounou

40. Assessing Transmission Blockade in Plasmodium spp.

Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden

41. Mosquito Transgenic Technologies to Reduce Plasmodium Transmission

Silke Fuchs, Tony Nolan, and Andrea Crisanti

Additional information

Malaria: Methods and Protocols by Robert Menard
Humana Press Inc.
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