Somatic Stem Cells: Methods and Protocols by Shree Ram Singh

Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.

Section I: Introduction

1. Current Thoughts on the Therapeutic Potential of Stem Cell

Pranela Rameshwar

Section II: Stem Cell Study in Lower Organism

2. A Unique FACS Method to Isolate Stem Cells in Planarian

Tetsutaro Hayashi and Kiyokazu Agata

3. Identification of Neural Stem Cells in the Drosophila Larval Brain

Mo Weng, Hideyuki Komori, and Cheng-Yu Lee

4. Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut

Shree Ram Singh, Manoj K. Mishra, Steven X. Hou, and Maduri Kango-Singh

^{Section III: Stem Cell Study in Murine and Human Model}

^{5. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells}

^{Daniel Croagh, Rick Redvers, Wayne A. Phillips, and Pritinder Kaur}

^{6. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine}

^{Gracz, A.D., Puthoff, B.J., Magness, S.T.}

^{7. Isolation and Characterization of Distal Lung Progenitor Cells}

^{Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason, Carla F. Kim, David Warburton}

^{8. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells}

^{Kristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller}

^{9. Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells}

^{Weihui Liu, Nan You, Kefeng Dou}

^{10. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases}

^{Laurent Lescaudron, C.Boyer, Virginie Bonnamain, KD. Fink, X. Leveque, J. Rossignol, V. Nerriere-Daguin, AC Malouet, F. Lelan, N.D Dey, D. Michel-Monigadon, M. Lu, I. Neveu, S. von Hoersten, P. Naveilhan and GL Dunbar}

^{11. Functional Identification of Neural Stem Cell-Derived Oligodendrocytes}

^{Sofia Grade,Fabienne Agasse, Liliana Bernardino, Joao O. Malva}

^{12. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization}

^{Abhik Bandyopadhyay, Qiaoxiang Dong and Lu-Zhe Sun}

^{13. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures}

^{Steven J. Greco, Shyam A. Patel, Pranela Rameshwar}

^{^{14. Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells}}

^{^{Susanne Grassel, Sabine Stoeckl, Zsuzsa Jenei-Lanzl}}

^{^{15. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue}}

^{^{Andrew C. Boquest and Philippe Collas}}

^{^{16. Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine}}

^{^{Michael S. Badowski and David T. Harris}}

^{17. Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells}

^{Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde}

^{18. Isolation and Characterization of Human Prostate Stem/Progenitor Cells}

^{Changyong Guo, Baohui Zhang, and Isla P. Garraway}

^{19. Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts}

^{Isotta Chimenti, Roberto Gaetani, Lucio Barile, Elvira Forte, Vittoria Ionta, Francesco Angelini, Giacomo Frati, Elisa Messina, Alessandro Giacomello.}

^{20. Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes}

^{Anke M. Smits, Angelique A. van Oorschot and Marie-Jose Goumans}

^{21. Isolation, Expansion and Characterization of Human Islet-Derived Progenitor Cells}

^{Mugdha V. Joglekarand Anandwardhan A. Hardikar}

^{22. Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli}

^{Stefania Bruno and Giovanni Camussi}

^{23. Endothelial Colony Forming Progenitor Cell Isolation and Expansion}

^{Nicole A. Hofmann, Andreas Reinisch and Dirk Strunk}

^{24. Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes}

^{Manabu Ohyama and Tetsuro Kobayashi}

^{25. Human Salivary Gland Stem Cells: Isolation, Propagation and Characterization}

^{Silke Schwarz and Nicole Rotter}

^{26. Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells}

^{Virginia Tirino, Francesca Paino and Gianpaolo Papaccio}

^{27. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin}

^{Hsing-I Huang and Chung-Zu Wu}

^{28. Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression}

^{Chao-Ling Yao and Shiaw-Min Hwang}

^{29. Genetic Modification of Mesenchymal Stem Cells}

^{Andreia Escosteguy Vargas, Melissa Medeiros Markoski, Andres Delgado Canedo, Flavia Helena da Silva and Nance Beyer Nardi}

^{30. Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells}

^{Melissa Camassola, Luisa Maria Gomes de Macedo Braga, Pedro Cesar Chagastelles and Nance Beyer Nardi}

^{^{31. In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells}}

^{^{Kenichi Miharada and Yukio Nakamura}}

^{32. Methods for Cancer Stem Cell Detection and Isolation}

^{Virginia Tirino, Vincenzo Desiderio, Francesca Paino and Gianpaolo Papaccio}

^{33. Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain}

^{Ashish K. Rehni, Thakur Gurjeet Singh, Mansi Chitkara, and I. S. Sandhu}

Somatic Stem Cells Shree Ram Singh

Somatic Stem Cells by Shree Ram Singh

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Somatic Stem Cells: Methods and Protocols by Shree Ram Singh

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