Enzyme Assays by Robert Eisenthal

Enzyme Assays by Robert Eisenthal

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

SKU	Unavailable
ISBN 13	9780199638208
ISBN 10	0199638209
Title	Enzyme Assays
Author	Robert Eisenthal
Series	Practical Approach Series
Condition	Unavailable
Binding Type	Paperback
Publisher	Oxford University Press
Year published	2002-04-04
Number of pages	302
Cover note	Book picture is for illustrative purposes only, actual binding, cover or edition may vary.