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Membrane Transport By Stephen A. Baldwin (School of Biochemistry and Molecular Biology, University of Leeds)

Membrane Transport

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Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.
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Membrane Transport Summary


Membrane Transport: A Practical Approach by Stephen A. Baldwin (School of Biochemistry and Molecular Biology, University of Leeds)

Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.

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Membrane Transport Reviews


'Stephen A.Baldwin has performed a service to the scientific community in compelling these technical approaches to membrane transport studies' Journal Cell Science

Table of Contents


Assay of membrane transport in cells and membrane vesicles ; Reconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulum ; The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins ; Expression of foreign transport proteins in yeast ; Baculovirus-mediated overexpression of transport proteins ; The amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins ; Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters ; Detection and analysis of glucose transporters using photolabelling techniques ; Computer prediction of transporter topology and structure ; Two-dimensional crystallization of membrane proteins

Additional information

GOR010069579
Membrane Transport: A Practical Approach by Stephen A. Baldwin (School of Biochemistry and Molecular Biology, University of Leeds)
Stephen A. Baldwin (School of Biochemistry and Molecular Biology, University of Leeds)
Practical Approach Series
Used - Very Good
Paperback
Oxford University Press
2000-09-01
342
0199637040
9780199637041
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a used book - there is no escaping the fact it has been read by someone else and it will show signs of wear and previous use. Overall we expect it to be in very good condition, but if you are not entirely satisfied please get in touch with us.