1. Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae
Ricardo Fuentes and Juan Fernandez
2. MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos
Karen Goossens, Luc Peelman, and Ann Van Soom
3. Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies
Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, and Luc Leyns
4. Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian
Agnieszka Rybak-Wolf and Jordi Solana
5. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae
Bernard Thisse and Christine Thisse
6. LNA-based in Situ Hybridization Detection of mRNAs in Embryos
Diana K. Darnell and Parker B. Antin
7. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA (TM) Probe Technology
Boye Schnack Nielsen, Trine Moller, and Kim Holmstrom
8. MicroRNA In Situ Hybridisation in Tissue Microarrays
Julia J. Turnock-Jones and John P.C. Le Quesne
9. Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections
Asli Silahtaroglu
10. Sensitive and Specific
in situ Hybridisation for Early Drug Discovery
Pernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum, and Kasper Almholt
11. Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections
Electra Stylianopoulou, George Skavdis and Maria Grigoriou
12. Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope
Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma
13. Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens
Lorenzo F. Sempere
14. Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues
Neil Renwick, Pavol Cekan, Claudia Bognanni, and Thomas Tuschl
15. Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris (R) RNA Fluorescence in situ Hybridization in Adherent Cells
Sally R. Coassin, Arturo V. Orjalo, Jr., Sheila J. Semaan, and Hans E. Johansson
16. Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope
Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma
17. Identification of Low Expressing Transcripts of the NPY Receptors' Family in the Murine Lingual Epithelia
Sergei Zolotukhin
18. In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Kathleen T. Montone
19. In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based Tissue Extraction from Thin Slide-mounted Sections for DNA Methylation Analysis
Lawrence S. Own and Paresh D. Patel
20. miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH
Meiye Wu, Matthew E. Piccini, and Anup K. Singh
21. PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples
Mustafa Fazli, Thomas Bjarnsholt, Niels Hoiby, Michael Givskov, and Tim Tolker-Nielsen
