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Rhinoviruses By David A. Jans

Rhinoviruses by David A. Jans

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Summary

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication.

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Rhinoviruses Summary

Rhinoviruses: Methods and Protocols by David A. Jans

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.

Table of Contents

1. Classification and Evolution of Human Rhinoviruses

Ann C. Palmenberg and James E. Gern

2. Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples

Xuelin Yu and Reena Ghildyal

3. Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples

Wai-Ming Lee, Kris Grindle, Rose Vrtis, Tressa Pappas, Fue Vang, Iris Lee, James E. Gern

4. Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing

Wei Wang, Jing He, Yi Liu, Lei Xu, Wencai Guan, and Yunwen Hu

5. Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions

Wai-Ming Lee, Yin Chen, Wensheng Wang, and Anne Mosser

6. Propagation of Rhinovirus - C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface

Shamaila Ashraf, Rebecca Brockman-Schneider, and James E. Gern

7. Infectivity Assays of Human Rhinovirus-A and -B Serotypes

Wai-Ming Lee, Yin Chen, Wensheng Wang, and Anne Mosser

8. Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating

Shushan Harutyunyan, Arthur Sedivy, Gottfried Koehler, Heinrich Kowalski, and Dieter Blaas

9. Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research

Victor U. Weiss, Xavier Subirats, Mohit Kumar, Shushan Harutyunyan, Irene Goesler, Heinrich Kowalski, and Dieter Blaas

10. Proteases of Human Rhinovirus - Role in Infection

Lora M. Jensen, Erin J. Walker, David A. Jans, and Reena Ghildyal

11. A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays

Lora M. Jensen, Erin J. Walker, and Reena Ghildyal

12. Reverse Genetics System for Studying Human Rhinovirus Infections

Wai-Ming Lee, Wensheng Wang, Yury A. Bochkov, and Iris Lee

13. Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag

Erin J. Walker, Lora M. Jensen, and Reena Ghildyal

14. Mouse Models of Rhinovirus Infection and Airways Disease

Nathan W. Bartlett, Aran Singanayagam, and Sebastian L. Johnston

Additional information

NLS9781493955336
9781493955336
1493955330
Rhinoviruses: Methods and Protocols by David A. Jans
New
Paperback
Humana Press Inc.
20160823
190
null null null null null null null null null null
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