Chapter 1: Historical overview and general methods of membrane potential imaging Lawrence B Cohen Chapter 2: Design and use of organic voltage sensitive dyes Leslie M Loew Chapter 3: Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines Marco Canepari, Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M Salzberg, Amiram Grinvald, Srdjan D Antic and Dejan Zecevic Chapter 4: Combined voltage and calcium imaging and signal calibration Marco Canepari, Peter Saggau and Dejan Zecevic Chapter 5: Use of fast-responding voltage-sensitive dyes for large-scale recording of neuronal spiking activity with single-cell resolution William N Frost, Jean Wang, Christopher J Brandon, Caroline Moore-Kochlacs, Terrence J Sejnowski and Evan S Hill Chapter 6: Monitoring integrated activity of individual neurons using FRET-based voltage-sensitive dyes Kevin L Briggman, William B Kristan, Jesus E Gonzalez, David Kleinfeld and Roger Y Tsien Chapter 7: Monitoring population membrane potential signals from neocortex Xiaoying Huang, Weifeng Xu, Kentaroh Takagaki and Jian-young Wu Chapter 8: Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos Yoko Momose-Sato, Katsushige Sato and Kohtaro Kamino Chapter 9: Imaging the dynamics of mammalian neocortical population activity in-vivo Amiram Grinvald, David Omer, Shmuel Naaman and Dahlia Sharon Chapter 10: Imaging the dynamics of neocortical population activity in behaving and freely moving mammals Amiram Grinvald and Carl CH Petersen Chapter 11: Monitoring membrane voltage using two-photon excitation of fluorescent voltage-sensitive dyes Jonathan AN Fisher and Brian M Salzberg Chapter 12: Random-access multiphoton microscopy for fast three-dimensional Imaging Gaddum Duemani Reddy and Peter Saggau Chapter 13: Second harmonic imaging of membrane potential Leslie M. Loew and Aaron Lewis Chapter 14: Genetically encoded protein sensors of membrane potential Lei Jin, Hiroki Mutoh, Thomas Knopfel, Lawrence B Cohen, Thom Hughes, Vincent A Pieribone, Ehud Y Isacoff, Brian M Salzberg, and Bradley J Baker