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Synthetic Messenger RNA and Cell Metabolism Modulation By Peter M. Rabinovich

Synthetic Messenger RNA and Cell Metabolism Modulation by Peter M. Rabinovich

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Synthetic Messenger RNA and Cell Metabolism Modulation Summary

Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols by Peter M. Rabinovich

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Table of Contents

Part I. mRNA Synthesis, Purification, and Transfer

1. Cell Engineering with Synthetic Messenger RNA

Peter M. Rabinovich and Sherman M. Weissman

2. In vitro Transcription of Long RNA Containing Modified Nucleosides

Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Kariko

3. HPLC Purification of in vitro Transcribed Long RNA

Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Kariko

4. Synthetic mRNAs with Superior Translation and Stability Properties

Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads

5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA

James G. Hecker

6. Light-Induced mRNA Transfection

Sigurd Leinaes Boe and Eivind Hovig

7. Engineering B Cells with mRNA

Jaewoo Lee, David Boczkowski, and Smita Nair

8. Programming Human Dendritic Cells with mRNA

Jaewoo Lee, David Boczkowski, and Smita Nair

9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process

Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa

10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

Melanie Galla, Axel Schambach, and Christopher Baum

11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells

Frank Breinig, Tanja Breinig, and Manfred J. Schmitt

Part 2. Modulation and monitoring of cell metabolism

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

Tobias Riet, Astrid Holzinger, Jan Doerrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

Noriko Shimasaki and Dario Campana

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

Peizhe Wang and Jie Na

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, OEzlem Tureci, and Ugur Sahin

16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

Chantal Pichon and Patrick Midoux

17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

OEzcan Met and Inge Marie Svane

18. mRNA electroporation as a Tool for Immunomonitoring

Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman

19. mRNA PCR-Based Epitope Chase Method

Jean-Daniel Doucet, Dominique Gauchat, and Rejean Lapointe

Part 2. Modulation and monitoring of cell metabolism

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

Tobias Riet, Astrid Holzinger, Jan Doerrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

Noriko Shimasaki and Dario Campana

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

Peizhe Wang and Jie Na

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, OEzlem Tureci, and Ugur Sahin

16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

Chantal Pichon and Patrick Midoux

17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

OEzcan Met and Inge Marie Svane

18. mRNA electroporation as a Tool for Immunomonitoring

Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman

19. mRNA PCR-Based Epitope Chase Method

Jean-Daniel Doucet, Dominique Gauchat, and Rejean Lapointe

Additional information

NLS9781493959679
9781493959679
1493959670
Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols by Peter M. Rabinovich
New
Paperback
Humana Press Inc.
20160823
323
null null null null null null null null null null
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