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Enhancers and Promoters Tilman Borggrefe

Enhancers and Promoters By Tilman Borggrefe

Enhancers and Promoters by Tilman Borggrefe

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Enhancers and Promoters Summary

Enhancers and Promoters: Methods and Protocols by Tilman Borggrefe

This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describeenhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays.Written in the Methods in Molecular Biologyseries format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting.

Authoritative and cutting-edge,Enhancers and Promoters: Methods and Protocolsis a useful guide for future experiments.
Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Table of Contents

A New Toolbox to Analyze Enhancer-Promoter Functions.-Global Run-on Sequencing (GRO-Seq).-Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq).-Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters.-Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq.-Measuring Chromatin Accessibility: ATAC-Seq.-High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.-Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.-Low Input Targeted Chromatin Capture (low-T2C).-Proximity Ligation-assisted ChIP-Seq (PLAC-Seq).-Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies.-Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions.-Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C).-Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.-Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin.-Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq).-Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.-Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors.-Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing.-Low Input Whole-Genome Bisulfite Sequencing.


Additional information

NPB9781071615997
9781071615997
1071615998
Enhancers and Promoters: Methods and Protocols by Tilman Borggrefe
Tilman Borggrefe
Methods in Molecular Biology
New
Paperback
Springer-Verlag New York Inc.
2022-08-14
376
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a new book - be the first to read this copy. With untouched pages and a perfect binding, your brand new copy is ready to be opened for the first time

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