1. Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture
Yuanwei Yan and Su-Chun Zhang
2. Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells
Peiyan Ni, Lingyi Fan, Amy Zinski, and Sangmi Chung
3. Generation and Co-Culture of Cortical Glutamatergic and GABAergic Induced Neuronal Cells
Jay English, Danny McSweeney, Fumiko Ribbe, Ethan Howell, and ChangHui Pak
4. Transcription Factor-Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Yi Han Ng and Justyna A. Janas
5. Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors
Shih-Wei Chen and Yu-Hui Wong
6. The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells
Mikael Lehoux, Kevin Connolly, Benedetta Assetta, and Yu-Wen Alvin Huang
7. Modeling Cellular Crosstalk of Neuroinflammation Axis by Tri-Cultures of iPSC-Derived Human Microglia, Astrocytes, and Neurons
Kevin Connolly, Mikael Lehoux, Benedetta Assetta, and Yu-Wen Alvin Huang
8. Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells
Zoe Mattingly and Sundari Chetty
9. Characterizing the Neuron-Glial Interactions by the Co-Cultures of Human iPSC-Derived Oligodendroglia and Neurons
Gabriella Vulakh and Xin Yang
10. Defined Differentiation of Human Pluripotent Stem Cells to Brain Microvascular Endothelial-Like Cells for Modeling the Blood-Brain Barrier
Koji L. Foreman, Eric V. Shusta, and Sean P. Palecek
11. Modeling the Blood-Brain Barrier Using Human Induced Pluripotent Stem Cells
Louise A. Mesentier-Louro, Natalie Suhy, Diede Broekaart, Michael Bula, Ana C. Pereira, and Joel W. Blanchard
12. A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally-Mediated Fluorescence, and Live Microscopy
Sophie Brown, Elaina Atherton, and David A. Borton
13. Method to Generate Dorsal Forebrain Brain Organoids from Human Pluripotent Stem Cells
Rebecca Sebastian, Narciso S. Pavon, Yoonjae Song, Karmen T. Diep, and ChangHui Pak
14. A 3D Bioengineered Neural Tissue Model Generated from Human iPSC-Derived Neural Precursor Cells
Selene Lomoio and Giuseppina Tesco
15. FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids
Liam Murray, Meagan N. Olson, Nathaniel Barton, Pepper Dawes, Yingleong Chan, and Elaine T. Lim
16. Dynamic Measurement of Endosome-Lysosome Fusion in Neurons Using High-Content Imaging
Qing Ouyang, Michael Schmidt, and Eric M. Morrow
17. Live-Imaging Detection of Multivesicular Body-Plasma Membrane Fusion and Exosome Release in Cultured Primary Neurons
Matthew F. Pescosolido, Qing Ouyang, Judy S. Liu, and Eric M. Morrow
18. Assays of Monitoring and Measuring Autophagic Flux for iPSC-Derived Human Neurons and Other Brain Cell Types
Ryan O'Rourke, Guzide Ayse Erdemir, and Yu-Wen Alvin Huang
19. Measuring Neuronal Network Activity Using Human Induced Neuronal Cells
Danny McSweeney, Jay English, Ethan Howell, Fumiko Ribbe, and ChangHui Pak
20. A Simple Ca2+-Imaging Approach of Network-Activity Analyses for Human Neurons
Zijun Sun
21. Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells
Rafael Gabriel III, Andrew J. Boreland, and Zhiping P. Pang
22. Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks
Deborah Pre, Alexander T. Wooten, Haowen Zhou, Ashley Neil, and Anne G. Bang
