Dora Goes to School/Dora Va a la Escuela (Dora the Explorer) by Random House

Dora Goes to School/Dora Va a la Escuela (Dora the Explorer) by Random House

If one were to design the ideal nuisance for cell cultures, the resultant might well be similar to mycoplasmas. These micro- organisms are very prevalent in nature, being found in the oral cavity, blood, the mucous membranes of the respiratory and uro- genital tract and other tissues of both man and animals. They are relatively difficult to detect microbiologically and chemically. Lacking cell walls, they do not routinely produce turbidity in cell cultures and are resistant to antibiotics that act on cell walls. Mycoplasmas grow to high titers in cell cultures. Concen- 7 8 trations of 10-10 colony forming units per ml of supernatant medium are representative. Additionally, more numbers are attached to cell membranes. Further, mycoplasmas have been shown to mimic in vitro effects of viruses and toxic chemicals. In various cell culture systems, mycoplasmas have been known to cause cell death, decrease or increase cell growth, affect virus tite s, induce interferon, cause chromosome damage, induce transformation, cyto- pathic effects, alter phenotypic expression, and significantly alter metabolic pathways and products of cells. The presence of such high concentrations of mycoplasmas in cell cultures constitutes a true in vitro infection. Such infected cell cultures, with a 8 9 total of 10 _10 or more actively metabolizing mycoplasmas, have no place in controlled, standardized cell culture procedures. Numerous reports have been published on mycoplasma assay pro- cedures, effects of infection, and preventive and elimination measures.

Established in July 2013 in a merger between Penguin and Random House, Penguin Random House, with nearly 250 independent imprints and brands on five continents, more than 15,000 new titles published each year, and close to 800 million print, audio, and eBooks sold annually, is the world's leading trade book publisher. Like its predecessor companies, Penguin Random House is committed to publishing adult and children's fiction and nonfiction print editions and is a pioneer in digital publishing. Its publishing lists include more than 60 Nobel Prize laureates and hundreds of the world's most widely read authors.

Jason Fruchter has been an animator and illustrator for children's media for decades. He graduated from the Rhode Island School of Design with a BFA in 1992, and later moved to New York City to work for MTV Animation. In 2001, he cofounded A&J Studios, and has worked with Cartoon Network, Disney, Nickelodeon, and Sanrio. His books include Callie's Cowgirl Twirl, Christmas Countdown, and Alphabet Magic. He lives in Seattle, Washington.

SKU	Unavailable
ISBN 13	9780385374965
ISBN 10	0385374968
Title	Dora Goes to School/Dora Va a la Escuela (Dora the Explorer)
Author	Random House
Condition	Unavailable
Binding Type	Paperback
Publisher	Random House USA Inc
Year published	2014-01-07
Number of pages	24
Cover note	Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
Note	Unavailable