Gene Isolation and Mapping Protocols by Jacqueline Boultwood

Gene Isolation and Mapping Protocols by Jacqueline Boultwood

Over the past 10 years great progress has been made in the development of efficient techniques for both gene isolation and mapping. The identifica- tion and isolation of transcribed sequences from large chromosomal regions are central to the human genome mapping project. Techniques for isolating novel cDNAs have applications both in the overall construction and integra- tion of long-range physical and transcription maps and in the identification of disease genes. A number of different techniques for the isolation of cDNAs from mam- malian genomes have been developed, including screening zoo blots, the use of large genomic clones (YACs or cosmids) for hybridization against cDNA libraries, and CpG island mapping. More recently two highly efficient tech- niques have been introduced: exon trapping, based on the presence of exon splice sites, and direct selection, based on the enrichment of selected cDNAs using immobilized YACs or cosmids. Leading researchers in the field have contributed chapters detailing the practical procedures for these and other widely used methods. The most rapid progress presently being made in the field of gene isolation concerns the partial sequencing of cDNA clones from one or both ends to produce expressed sequence tags (ESTs). Indeed, by Octo- ber 1995, the EST division of Genbank (dbEST) contained a total of approxi- mately 270,000 human EST sequences accounting for almost half the number of sequence entries in Genbank.

..a useful addition to the libraries of individuals and institutions... Three Stars. - Doody's Health Sciences Book Review Journal

"...this series of excellent and detailed lab manuals....of general use to all those mapping and isolation genes."-SGM Quarterly

SKU	Unavailable
ISBN 13	9780896033825
ISBN 10	0896033821
Title	Gene Isolation and Mapping Protocols
Author	Jacqueline Boultwood
Series	Methods In Molecular Biology
Condition	Unavailable
Binding Type	Paperback
Publisher	Humana Press Inc.
Year published	1996-10-19
Number of pages	318
Cover note	Book picture is for illustrative purposes only, actual binding, cover or edition may vary.