Immunoassays by Gosling

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Summary

Pregnancy tests, HIV tests and tests to confirm heart attacks and many other clinical diagnostic tests are all immunoassays. Without immunoassays a huge variety of biologically important molecules could not be easily detected or quantified. Immunoassays will help any life scientist develop his/her own immunoassay.

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Immunoassays by Gosling

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
"This book is very useful not only for immunologists but also for amny specialists in chemistry and biology and is certainly recommendable" Immunological Investigations
SKU Unavailable
ISBN 13 9780199637102
ISBN 10 0199637105
Title Immunoassays
Author Gosling
Series Practical Approach Series
Condition Unavailable
Binding Type Paperback
Publisher Oxford University Press
Year published 2000-08-31
Number of pages 328
Cover note Book picture is for illustrative purposes only, actual binding, cover or edition may vary.