Part I: Enhanced Approaches to Inherent Problems
1. Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis
Hiroyuki Takahara, Elmar Endl, and Richard O'Connell
2. Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA
Hiroshi Nojima and Takahiro Tougan
3. Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening
Chaofu Lu, James G. Wallis, and John Browse
4. Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method
Seishi Kato, Mio Oshikawa, and Kuniyo Ohtoko
5. Construction of Improved Yeast Two-Hybrid Libraries
Richard H. Maier, Christina J. Maier, and Kamil OEnder
6. Normalization of Full-Length Enriched cDNA
Ekaterina A. Bogdanova, Ekaterina V. Barsova, Irina A. Shagina, Alexander Scheglov, Veronika Anisimova, Laura L. Vagner, Sergey A. Lukyanov, and Dmitry A. Shagin
7. Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets
John M.J. Herbert, Dov J. Stekel, Manuela Mura, Michail Sychev, and Roy Bicknell
Part II: Visionary Applications
8. Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs
Kohtaroh Sugao and Kenzo Hirose
9. Construction of Small RNA cDNA Libraries for High-Throughput Sequencing
Cheng Lu and Vikas Shedge
10. Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO)
Andreas Gratz and Joachim Jose
11. Generation of Families of Construct Variants Using Golden Gate Shuffling
Carola Engler and Sylvestre Marillonnet
12. Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function
Youichi Kondou, Mieko Higuchi, Takanari Ichikawa, and Minami Matsui
13. Construction of Yeast Surface-Displayed cDNA Libraries
Scott Bidlingmaier and Bin Liu
14. Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries
Scott Bidlingmaier and Bin Liu
15. SNP Discovery by Transcriptome Pyrosequencing
W. Brad Barbazuk and Patrick S. Schnable
16. RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy
Michael T. Lovci, Hai-Ri Li, Xiang-dong Fu, and Gene W. Yeo
17. Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species
Diana Bellin, Alberto Ferrarini, and Massimo Delledonne
