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Proteins Gary Walsh

Proteins By Gary Walsh

Proteins by Gary Walsh


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Summary

Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine.

Proteins Summary

Proteins: Biochemistry and Biotechnology by Gary Walsh

Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine. Fully updated throughout with new or fundamentally revised sections on proteomics as, bioinformatics, protein glycosylation and engineering, well as sections detailing advances in upstream processing and newer protein applications such as enzyme-based biofuel production this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies. This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology Includes numerous case studies Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production "With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. " ( Engineering in Life Sciences, 2004; Vol 5; No. 5) Few texts would be considered competitors, and none compare favorably." ( Biochemistry and Molecular Education, July/August 2002) "...The book is well written, making it informative and easy to read..." ( The Biochemist, June 2002)

About Gary Walsh

Gary Walsh, University of Limerick, Ireland.

Table of Contents

Preface xi About the Companion Website xiii Chapter 1 Proteins and proteomics 1 1.1 Proteins, an introduction 1 1.2 Genes, genomics and proteomics 2 1.3 Bioinformatics 12 1.4 Proteomics: goals and applications 14 Further reading 22 Chapter 2 Protein structure and engineering 25 2.1 Primary structure 25 2.2 Higher-level structure 36 2.3 Protein classification on the basis of structure 41 2.4 Protein structural stability 45 2.5 Higher-order structure prediction 47 2.6 Protein folding 48 2.7 Intrinsically disordered proteins 50 2.8 Protein engineering 51 2.9 Protein post-translational modification 54 Further reading 62 Chapter 3 Protein sources 65 3.1 Recombinant versus non-recombinant production 65 3.2 Approaches to recombinant protein production 67 3.3 Heterologous protein production in E. coli 72 3.4 Heterologous production in bacteria other than E. coli 77 3.5 Heterologous protein production in yeast 77 3.6 Heterologous protein production in fungi 78 3.7 Proteins from plants 80 3.8 Animal tissue as a protein source 84 3.9 Heterologous protein production in transgenic animals 85 3.10 Heterologous protein production using animal cell culture 86 3.11 Insect cell culture systems 87 Chapter 4 Protein purification and characterization 91 4.1 Protein detection and quantification 93 4.2 Initial recovery of protein 95 4.3 Removal of whole cells and cell debris 98 4.4 Concentration 103 4.5 Chromatographic purification 107 4.6 Protein inactivation and stabilization 128 4.7 Protein characterization 137 Further reading 139 Chapter 5 Large-scale protein production 141 5.1 Upstream processing 141 5.2 Downstream processing 154 5.3 Therapeutic protein production: some special issues 163 5.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166 Further reading 175 Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 177 6.1 Blood products 177 6.2 Anticoagulants 184 6.3 Thrombolytic agents 186 6.4 Additional blood-related products 189 6.5 Vaccine technology 190 6.6 Therapeutic enzymes 194 Further reading 202 Chapter 7 Therapeutic antibodies 205 7.1 Antibodies 205 7.2 IgG structure and activity 205 7.3 Antibody therapeutics: polyclonal antibody preparations 209 7.4 Antibody therapeutics: monoclonal antibodies 211 7.5 Therapeutic applications of monoclonal antibodies 220 7.6 Antibody conjugates 223 7.7 Bispecific antibodies 224 7.8 Antibody fragments 225 7.9 Engineering the antibody glycocomponent 228 7.10 Fc fusion proteins 229 Further reading 230 Chapter 8 Hormones and growth factors used therapeutically 233 8.1 Insulin 233 8.2 Glucagon 240 8.3 Gonadotrophins 240 8.4 Growth hormone 243 8.5 Erythropoietin 246 8.6 Other hormones 247 8.7 Growth factors 249 Further reading 253 Chapter 9 Interferons, interleukins and tumour necrosis factors 257 9.1 Regulatory factors: cytokines versus hormones 257 9.2 Interferons 258 9.3 Interleukins 264 9.4 Tumour necrosis factors 271 Further reading 274 Chapter 10 Proteins used for analytical purposes 277 10.1 The IVD sector 279 10.2 The basis of analyte detection and quantification 280 10.3 Enzymes as diagnostic/analytical reagents 281 10.4 Biosensors 289 10.5 Antibodies as analytical reagents 295 Further reading 309 Chapter 11 Industrial enzymes: an introduction 311 11.1 Sales value and manufacturers 313 11.2 Sources and engineering 314 11.3 Environmental benefits 315 11.4 Enzyme detection and quantification 315 11.5 Immobilized enzymes 316 11.6 Extremophiles 319 11.7 Enzymes in organic solvents 324 11.8 Industrial enzymes: the future 325 Further reading 325 Chapter 12 Industrial enzymes: proteases and carbohydrases 327 12.1 Proteolytic enzymes 327 12.2 Carbohydrases 340 Further reading 367 Chapter 13 Additional industrial enzymes 371 13.1 Lipases 371 13.2 Penicillin acylase 375 13.3 Amino acylase and amino acid production 378 13.4 Cyclodextrins and cyclodextrin glycosyltransferase 380 13.5 Enzymes and animal nutrition 382 13.6 Enzymes in molecular biology 387 Further reading 390 Chapter 14 Non-catalytic industrial proteins 393 14.1 Functional properties of proteins 393 14.2 Milk and milk proteins 397 14.3 Animal-derived proteins 408 14.4 Plant-derived proteins 411 14.5 Sweet and taste-modifying proteins 412 Further reading 414 Index 417

Additional information

GOR013643785
9780470669853
0470669853
Proteins: Biochemistry and Biotechnology by Gary Walsh
Used - Good
Paperback
John Wiley & Sons Inc
2014-05-13
446
N/A
Book picture is for illustrative purposes only, actual binding, cover or edition may vary.
This is a used book - there is no escaping the fact it has been read by someone else and it will show signs of wear and previous use. Overall we expect it to be in good condition, but if you are not entirely satisfied please get in touch with us

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