Chikungunya Virus: Methods and Protocols by Justin Jang Hann Chu

This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus.

Part I Clinical and Diagnostic Virology

1. Evolution and Epidemiology of Chikungunya Virus
Giada Rossini, Maria Paola Landini, and Vittorio Sambri

2. Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
Seok Mui Wang, Ummul Haninah Ali, Shamala Devi Sekaran, and Ravindran Thayan

3. Advanced Genetic Methodologies in Tracking Evolution and Spread of Chikungunya Virus
Hapuarachchige Chanditha Hapuarachchi and Kim-Sung Lee

4. Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications
Rajpal S. Kashyap, Shradha S. Bhullar, Nitin H. Chandak, and Girdhar M. Taori

5. Expression and Purification of E2 Glycoprotein from Insect Cells (Sf9) for Use in Serology
Chong Long Chua, I-Ching Sam, and Yoke Fun Chan

6. Diagnostic Methods for CHIKV Based on Serological Tools
Paolo Gaibani, Maria Poala Landini, and Vittorio Sambri

7. Utilization and Assessment of Throat Swab and Urine Specimens for Diagnosis of Chikungunya Virus Infection
Chandrashekhar G. Raut, H. Hanumaiah, and Wrunda C. Raut

Part II Cell Culture, Virus Replication, and Cellular Responses

8. Propagation of Chikungunya Virus Using Mosquito Cells
Swee Kim Ang, Shirley Lam, and Justin Jang Hann Chu

9. Infectious Viral Quantification of Chikungunya Virus - Virus Plaque Assay
Parveen Kaur, Regina Ching Hua Lee, and Justin Jang Hann Chu

10. Molecular Epidemiology of Chikungunya Virus by Sequencing
Ravindran Thayan, Mohd Apandi Yusof, Zainah Saat, Shamala Devi Sekaran, and Seok Mui Wang

11. Chikungunya Virus Infection of Aedes Mosquitoes
Hui Vern Wong, Yoke Fun Chan, I-Ching Sam, Wan Yusof Wan Sulaiman, and Indra Vythilingam

12. Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal
Jeremy P. Ledermann and Ann M. Powers

13. Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence Assay
Meng Ling Moi and Tomohiko Takasaki

14. Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
Chang-Kweng Lim

15. Viral-Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method
Namrata Dudha and Sanjay Gupta

16. Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection: Preparation of Peptides for Analysis
Atchara Paemanee, Nitwara Wikan, Sittiruk Roytrakul, and Duncan R. Smith

17. Bioinformatics Based Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection
Sreejith Rajasekharan and Sanjay Gupta

18. T-Cell Epitope Prediction of Chikungunya Virus
Christine Loan Ping Eng, Tin Wee Tan, and Joo Chuan Tong

Part III Immunology and Animal Model Studies

19. Mouse Models of Chikungunya Virus
Lara J. Herrero, Penny A. Rudd, Xiang Liu, Stefan Wolf, and Suresh Mahalingam

20. Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit
Chow Wenn Yew and Yee Joo Tan

21. Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues
Chun Wei Chiam, I-Ching Sam, Yoke Fun Chan, Kum Thong Wong, and Kien Chai Ong

Part IV Antivirals and Vaccines

22. Antiviral Strategies against Chikungunya Virus
Rana Abdelnabi, Johan Neyts, and Leen Delang

23. A Real Time Cell Analyzing Assay for Identification of Novel Antiviral Compounds against Chikungunya Virus
Keivan Zandi

24. Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compounds that Interfere the Infection of Chikungunya Virus
Szu-Cheng Kuo, Chao-Yi Teng, Yi-Jung Ho, Ying-Ju Chen, and Tzong-Yuan Wu

25. Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
Nor Azila Muhammad Azami, Meng Ling Moi, and Tomohiko Takasaki

26. Reverse Genetics Approaches for Chikungunya Virus
Patchara Phuektes and Justin Jang Hann Chu

27. Production of Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells
Stefan W. Metz and Gorben P. Pijlman

28. Protocols for Developing Novel Chikungunya Virus DNA Vaccines
Christopher Chung, Kenneth E. Ugen, Niranjan Y. Sardesai, David B. Weiner, and Kar Muthumani

Chikungunya Virus Justin Jang Hann Chu

Chikungunya Virus by Justin Jang Hann Chu

Chikungunya Virus Summary

Chikungunya Virus: Methods and Protocols by Justin Jang Hann Chu

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